验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, human liver tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| CoIP | See 1 publications below |
产品信息
15650-1-AP targets MTO1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | MTO1 fusion protein Ag8192 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial translation optimization 1 homolog (S. cerevisiae) |
| 别名 | Protein MTO1 homolog, mitochondrial, CGI-02, CGI 02 |
| 计算分子量 | 717 aa, 80 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC005808 |
| 基因名称 | MTO1 |
| Gene ID (NCBI) | 25821 |
| RRID | AB_2266805 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2Z2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTO1 antibody 15650-1-AP | Download protocol |
| IF protocol for MTO1 antibody 15650-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Restoration of mitochondrial function through activation of hypomodified tRNAs with pathogenic mutations associated with mitochondrial diseases | ||
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
Nucleic Acids Res The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. | ||
J Hepatol Severe respiratory complex III defect prevents liver adaptation to prolonged fasting. | ||
Am J Hum Genet Mutations of the mitochondrial-tRNA modifier MTO1 cause hypertrophic cardiomyopathy and lactic acidosis. | ||
Hum Mol Genet Altered 2-thiouridylation impairs mitochondrial translation in reversible infantile respiratory chain deficiency. |
