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MTMR4 Polyclonal antibody

MTMR4 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13488-1-AP

Print datasheet

Synonyms

FYVE DSP2, KIAA0647, MTMR4, myotubularin related protein 4, ZFYVE11

验证数据展示

  • WB analysis using 13488-1-AP

    WB analysis using 13488-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13488-1-AP (MTMR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13488-1-AP

    IF Staining of HepG2 using 13488-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (13488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13488-1-AP targets MTMR4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MTMR4 fusion protein Ag4310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myotubularin related protein 4
Synonyms FYVE DSP2, KIAA0647, MTMR4, myotubularin related protein 4, ZFYVE11
Calculated Molecular Weight 1195 aa, 133 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberBC035609
Gene Symbol MTMR4
Gene ID (NCBI) 9110
RRIDAB_2251008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).

实验方案

Product Specific Protocols
WB protocol for MTMR4 antibody 13488-1-APDownload protocol
IF protocol for MTMR4 antibody 13488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Biol Chem

PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking.

Authors - Dyaningtyas Dewi Pamungkas Putri
View Article