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验证数据展示

  • WB analysis of HeLa using 14372-1-AP

    WB analysis of HeLa using 14372-1-AP

    WB result of MTMR2 antibody (14372-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR2 transfected HeLa cells.

  • WB analysis using 14372-1-AP

    WB analysis using 14372-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse cerebellum using 14372-1-AP

    IP experiment of mouse cerebellum using 14372-1-AP

    IP result of anti-MTMR2 (IP:14372-1-AP, 4ug; Detection:14372-1-AP 1:300) with mouse cerebellum tissue lysate 4000 ug.

  • IHC staining of human endometrial cancer using 14372-1-AP

    IHC staining of human endometrial cancer using 14372-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 14372-1-AP

    IHC staining of human endometrial cancer using 14372-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

MTMR2 Polyclonal antibody

MTMR2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14372-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human cerebellum tissue, NIH/3T3 cells
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inhuman endometrial cancer tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14372-1-AP targets MTMR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5745

Product name: Recombinant human MTMR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 294-643 aa of BC052990

Sequence: KRSKEDEKYLQAIMDSNAQSHKIFIFDARPSVNAVANKAKGGGYESEDAYQNAELVFLDIHNIHVMRESLRKLKEIVYPNIEETHWLSNLESTHWLEHIKLILAGALRIADKVESGKTSVVVHCSDGWDRTAQLTSLAMLMLDGYYRTIRGFEVLVEKEWLSFGHRFQLRVGHGDKNHADADRSPVFLQFIDCVWQMTRQFPTAFEFNEYFLITILDHLYSCLFGTFLCNSEQQRGKENLPKRTVSLWSYINSQLEDFTNPLYGSYSNHVLYPVASMRHLELWVGYYIRWNPRMKPQEPIHNRYKELLAKRAELQKKVEELQREISNRSTSSSERASSPAQCVTPVQTVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myotubularin related protein 2
别名 CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2
计算分子量 73 kDa
观测分子量 66-73 kDa
GenBank蛋白编号BC052990
基因名称 MTMR2
Gene ID (NCBI) 8898
RRIDAB_2282336
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13614
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa.

实验方案

Product Specific Protocols
WB protocol for MTMR2 antibody 14372-1-APDownload protocol
IHC protocol for MTMR2 antibody 14372-1-APDownload protocol
IP protocol for MTMR2 antibody 14372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols