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验证数据展示

  • WB analysis of HeLa using 14372-1-AP

    WB analysis of HeLa using 14372-1-AP

    WB result of MTMR2 antibody (14372-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR2 transfected HeLa cells.

  • WB analysis using 14372-1-AP

    WB analysis using 14372-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse cerebellum using 14372-1-AP

    IP experiment of mouse cerebellum using 14372-1-AP

    IP result of anti-MTMR2 (IP:14372-1-AP, 4ug; Detection:14372-1-AP 1:300) with mouse cerebellum tissue lysate 4000 ug.

  • IHC staining of human endometrial cancer using 14372-1-AP

    IHC staining of human endometrial cancer using 14372-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 14372-1-AP

    IHC staining of human endometrial cancer using 14372-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

MTMR2 Polyclonal antibody

MTMR2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14372-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human cerebellum tissue, NIH/3T3 cells
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inhuman endometrial cancer tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14372-1-AP targets MTMR2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MTMR2 fusion protein Ag5745 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myotubularin related protein 2
别名 CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2
计算分子量 73 kDa
观测分子量 66-73 kDa
GenBank蛋白编号BC052990
基因名称 MTMR2
Gene ID (NCBI) 8898
RRIDAB_2282336
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13614
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa.

实验方案

Product Specific Protocols
WB protocol for MTMR2 antibody 14372-1-APDownload protocol
IHC protocol for MTMR2 antibody 14372-1-APDownload protocol
IP protocol for MTMR2 antibody 14372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols