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验证数据展示

  • WB analysis using 83372-7-RR (same clone as 83372-7-PBS)

    WB analysis using 83372-7-RR (same clone as 83372-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83372-7-RR (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83372-7-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83372-7-RR (same clone as 83372-7-PBS)

    FC experiment of HeLa using 83372-7-RR (same clone as 83372-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTHFR Recombinant antibody (83372-7-RR, Clone:240348C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83372-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83372-7-RR

    Affinity and Kinetic Characterization of 83372-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83372-7-RR against Human MTHFR were performed. The affinity constant is below 1 pM.

MTHFR Recombinant monoclonal antibody, PBS Only

MTHFR Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83372-7-PBS

产品说明书

CloneNo. 240348C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

240348C12, EC:1.5.1.53, Methylenetetrahydrofolate reductase (NADPH)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83372-7-PBS targets MTHFR in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14970

Product name: Recombinant human MTHFR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 307-656 aa of BC053509

Sequence: QELLASGLVPGLHFYTLNREMATTEVLKRLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEASVFEVFVLYLSGEPNRNGHKVTCLPWNDEPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVEDTLELLNRPTQNARETEAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5,10-methylenetetrahydrofolate reductase (NADPH)
别名 240348C12, EC:1.5.1.53, Methylenetetrahydrofolate reductase (NADPH)
计算分子量 656 aa, 75 kDa
GenBank蛋白编号BC053509
基因名称 MTHFR
Gene ID (NCBI) 4524
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP42898
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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