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验证数据展示

  • WB analysis of LNCaP using 66612-1-Ig (same clone as 66612-1-PBS)

    WB analysis of LNCaP using 66612-1-Ig (same clone as 66612-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66612-1-Ig (same clone as 66612-1-PBS)

    IHC staining of human liver using 66612-1-Ig (same clone as 66612-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66612-1-Ig (same clone as 66612-1-PBS)

    IF Staining of HeLa using 66612-1-Ig (same clone as 66612-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTHFR antibody (66612-1-Ig, Clone: 1F7A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

MTHFR Monoclonal antibody, PBS Only

MTHFR Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66612-1-PBS

产品说明书

CloneNo. 1F7A3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 1F7A3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66612-1-PBS targets MTHFR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 MTHFR fusion protein Ag14970 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 5,10-methylenetetrahydrofolate reductase (NADPH)
别名 Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 1F7A3
计算分子量 656 aa, 75 kDa
观测分子量 70 kDa
GenBank蛋白编号BC053509
基因名称 MTHFR
Gene ID (NCBI) 4524
RRIDAB_2881972
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42898
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

5,10-Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in the folate metabolic pathway with a key role in generating methyl groups. MTHFR reduces 5,10-methylenetetrahydrofolate (5,10-methyleneTHF) to 5-methylTHF, the primary carbon donor for methionine production from homocysteine. In humans and mice studies, mutations and polymorphisms in MTHFR are associated with impaired spermatogenesis and male infertility. It has been reported that MTHFR is highly phosphorylated under unperturbed conditions and T34 is the priming phosphorylation site. MTHFR gene has two promoters and isoform (70 kDa and 77 kDa). Although the major form of MTHFR is 77kDa in most tissues, a second isoform of 70kDa was observed after western blotting of human fetal liver and porcine liver. (PMID: 24769206, PMID: 34128976, PMID: 18615588)