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  • KD/KO Validated

MTHFD1 Polyclonal antibody

MTHFD1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10794-1-AP

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Synonyms

MTHFC, MTHFD, MTHFD1



经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, HuH-7 cells, K-562 cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MTHFD1 Ready-To-Use IHC Kit. MTHFD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10794-1-AP targets MTHFD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen MTHFD1 fusion protein Ag1225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Synonyms MTHFC, MTHFD, MTHFD1
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC009806
Gene Symbol MTHFD1
Gene ID (NCBI) 4522
RRIDAB_2147391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

实验方案

Product Specific Protocols
WB protocol for MTHFD1 antibody 10794-1-APDownload protocol
IHC protocol for MTHFD1 antibody 10794-1-APDownload protocol
IP protocol for MTHFD1 antibody 10794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Oxidative stress inhibits distant metastasis by human melanoma cells.

Authors - Elena Piskounova
  • KD Validated
humanWB

J Pineal Res

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Authors - Li Cui
humanWB

Redox Biol

Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.

Authors - Chang Shao
humanWB

Proc Natl Acad Sci U S A

Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.

Authors - Danielle E Anderson
  • KD Validated
mouseWB

Dev Cell

Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.

Authors - Yejun Zou
humanWB

Cell Death Dis

MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified neuroblastoma

Authors - Jinqiu Guan