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验证数据展示

  • WB analysis of human brain using 13742-1-AP

    WB analysis of human brain using 13742-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13742-1-AP (MTG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 13742-1-AP

    IF Staining of SH-SY5Y using 13742-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 13742-1-AP (MTG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MTG1 Polyclonal antibody

MTG1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13742-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Mitochondrial ribosome-associated GTPase 1, Mitochondrial GTPase 1, GTPBP7, GTP-binding protein 7, GTP binding protein 7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, human liver tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13742-1-AP targets MTG1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag4681

Product name: Recombinant human MTG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC035721

Sequence: MAKGLKKMQSSLKLVDCIIEVHDARIPLSGRNPLFQETLGLKPHLLVLNKMDLADLTEQQKIMQHLEGEGLKNVIFTNCVKDENVKQIIPMVTELIGRSHRYHRKENLEYCIMVIGVPNVGKSSLINSLRRQHLRKGKATRVGGEPGITRAVMSKIQVSERPLMFLLDTPGVLAPRIESVETGLKLALCGTVLDHLVGEETMADYLLYTLNKHQRFGYVQHYGLGSACDNVERVLKSVAVKLGKTQKVKVLTGTGNVNVIQPNYPAAARDFLQTFRRGLLGSVMLDLDVLRGHPPAETLP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial GTPase 1 homolog (S. cerevisiae)
别名 Mitochondrial ribosome-associated GTPase 1, Mitochondrial GTPase 1, GTPBP7, GTP-binding protein 7, GTP binding protein 7
计算分子量 300 aa, 33 kDa
观测分子量 37 kDa
GenBank蛋白编号BC035721
基因名称 MTG1
Gene ID (NCBI) 92170
RRIDAB_2147380
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BT17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for MTG1 antibody 13742-1-APDownload protocol
WB protocol for MTG1 antibody 13742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia
View Article
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
View Article
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