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验证数据展示

  • WB analysis using 83132-7-RR

    WB analysis using 83132-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of 143B.TK.P0 using 83132-7-RR

    WB analysis of 143B.TK.P0 using 83132-7-RR

    WB result of MTCO2 antibody (MTCO2; 1:3000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

  • IP experiment of HeLa using 83132-7-RR

    IP experiment of HeLa using 83132-7-RR

    IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug.

  • IHC staining of human ovarian cancer using 83132-7-RR

    IHC staining of human ovarian cancer using 83132-7-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTCO2/COX2 antibody included
  • IHC staining of human stomach cancer using 83132-7-RR

    IHC staining of human stomach cancer using 83132-7-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83132-7-RR (MTCO2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with MTCO2/COX2 antibody included
  • IF Staining of HepG2 using 83132-7-RR

    IF Staining of HepG2 using 83132-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83132-7-RR

    FC experiment of HeLa using 83132-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human MTCO2 (83132-7-RR, Clone:230448C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

MTCO2 Recombinant antibody

MTCO2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 83132-7-RR

产品说明书

CloneNo. 230448C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

COX2, MT CO2, COXII, COX 2, COII

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, 143B.TK.P0 cells, 143B cells, HeLa cells, U-251 cells, A549 cells, MDA-MB-231 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovarian cancer, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MTCO2/COX2 Ready-To-Use IHC Kit. MTCO2/COX2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83132-7-RR targets MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome c oxidase II
别名 COX2, MT CO2, COXII, COX 2, COII
计算分子量 26 kDa
观测分子量 23-26 kDa
GenBank蛋白编号YP_003024029
基因名称 MTCO2
Gene ID (NCBI) 4513
RRIDAB_3670835
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00403
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).

实验方案

Product Specific Protocols
WB protocol for MTCO2 antibody 83132-7-RRDownload protocol
IHC protocol for MTCO2 antibody 83132-7-RRDownload protocol
IF protocol for MTCO2 antibody 83132-7-RRDownload protocol
IP protocol for MTCO2 antibody 83132-7-RRDownload protocol
FC protocol for MTCO2 antibody 83132-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols