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MTA2 Monoclonal antibody
MTA2 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2G12G4
验证数据展示
经过测试的应用
Positive WB detected in | SW480 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IHC detected in | mouse brain tissue, human lung tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:125-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66195-1-Ig targets MTA2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | MTA2 fusion protein Ag11713 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | metastasis associated 1 family, member 2 |
Synonyms | Metastasis associated 1 like 1, MTA1 L1 protein, MTA1L1, MTA2, PID |
Calculated Molecular Weight | 668 aa, 75 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC053650 |
Gene Symbol | MTA2 |
Gene ID (NCBI) | 9219 |
RRID | AB_2877118 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O94776 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MTA2 antibody 66195-1-Ig | Download protocol |
IHC protocol for MTA2 antibody 66195-1-Ig | Download protocol |
IF protocol for MTA2 antibody 66195-1-Ig | Download protocol |
FC protocol for MTA2 antibody 66195-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Dev Cell Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells | ||
Theranostics LncRNA-MTA2TR functions as a promoter in pancreatic cancer via driving deacetylation-dependent accumulation of HIF-1α.
| ||
Oncol Lett Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells. | ||
Cancers (Basel) ZEB2/TWIST1/PRMT5/NuRD Multicomplex Contributes to the Epigenetic Regulation of EMT and Metastasis in Colorectal Carcinoma | ||
Nat Chem Biol ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism
| ||
Cell Chem Biol HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation |