验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, HT-1080 cells, rat kidney tissue |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86358-1-RR targets MSRA in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag6053 Product name: Recombinant human MSRA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC054033 Sequence: MLSATRRACQLLLLHSLFPVPRMGNSASNIVSPQEALPGRKEQTPVAAKHHVNGNRTVEPFPEGTQMAVFGMGCFWGAERKFWVLKGVYSTQVGFAGGYTSNPTYKEVCSEKTGHAEVVRVVYQPEHMSFEELLKVFWENHDPTQGMRQGNDHGTQYRSAIYPTSAKQMEAALSSKENYQKVLSEHGFGPITTDIREGQTFYYAEDYHQQYLSKNPNGYCGLGGTGVSCPVGIKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | methionine sulfoxide reductase A |
| 别名 | EC:1.8.4.11, Mitochondrial peptide methionine sulfoxide reductase, Peptide Met(O) reductase, Peptide-methionine, PMSR |
| 计算分子量 | 26 kDa |
| 观测分子量 | 24-26 kDa |
| GenBank蛋白编号 | BC054033 |
| 基因名称 | MSRA |
| Gene ID (NCBI) | 4482 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UJ68 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Methionine sulfoxide reductase A (MSRA) is an antioxidant enzyme found in all domains of life that catalyzes the reduction of methionine-S-sulfoxide (MSO) to methionine in proteins and free amino acids (PMID: 28874471). MSRA has some isoforms with MW of 19~26 kDa. MSRA functions in the repair of oxidatively damaged proteins to restore biological activity. Transfection of MSRA reduced colony formation and the invasiveness of HCC cell lines. Studies have demonstrated the downregulation of MSRA in multiple human tumors, including breast cancers and its levels have been linked to advanced tumor grades (PMID: 20937881).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MSRA antibody 86358-1-RR | Download protocol |
| WB protocol for MSRA antibody 86358-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
