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验证数据展示

  • WB analysis using 82009-1-RR

    WB analysis using 82009-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82009-1-RR (MSN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82009-1-RR

    WB analysis of HeLa using 82009-1-RR

    WB result of Moesin antibody (82009-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Moesin transfected HeLa cells.

  • IHC staining of human placenta using 82009-1-RR

    IHC staining of human placenta using 82009-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSN antibody included
  • IHC staining of mouse kidney using 82009-1-RR

    IHC staining of mouse kidney using 82009-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSN antibody included
  • IHC staining of mouse kidney using 82009-1-RR

    IHC staining of mouse kidney using 82009-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82009-1-RR (Moesin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSN antibody included
  • IF Staining of HepG2 using 82009-1-RR

    IF Staining of HepG2 using 82009-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Moesin antibody (82009-1-RR, Clone: 3B8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82009-1-RR

    Affinity and Kinetic Characterization of 82009-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82009-1-RR against Human MSN were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

Moesin Recombinant antibody

Moesin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82009-1-RR

产品说明书

CloneNo. 3B8

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MSN, Membrane-organizing extension spike protein, 3B8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, SGC-7901 cells, NK-92 cells, Jurkat cells, C6 cells, RAW 264.7 cells
Positive IHC detected inmouse kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MSN Ready-To-Use IHC Kit. MSN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82009-1-RR targets Moesin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Moesin fusion protein Ag9623 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 moesin
别名 MSN, Membrane-organizing extension spike protein, 3B8
计算分子量 577 aa, 68 kDa
观测分子量 68-70 kDa
GenBank蛋白编号BC017293
基因名称 Moesin
Gene ID (NCBI) 4478
RRIDAB_2935596
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP26038
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

实验方案

Product Specific Protocols
WB protocol for Moesin antibody 82009-1-RRDownload protocol
IHC protocol for Moesin antibody 82009-1-RRDownload protocol
IF protocol for Moesin antibody 82009-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols