MSH3 Recombinant antibody
MSH3 Recombinant Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1J23
验证数据展示
经过测试的应用
Positive WB detected in | HSC-T6 cells |
Positive IF detected in | U-251 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:750-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82845-3-RR targets MSH3 in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, WB, ELISA |
Tested Reactivity | Human |
Immunogen | MSH3 fusion protein Ag18060 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | mutS homolog 3 (E. coli) |
Synonyms | Divergent upstream protein, DUC1, DUG, DUP, hMSH3, Mismatch repair protein 1, MRP1, MSH3, mutS homolog 3 (E. coli) |
Calculated Molecular Weight | 1137 aa, 127 kDa |
Observed Molecular Weight | 127 kDa |
GenBank Accession Number | BC130434 |
Gene Symbol | MSH3 |
Gene ID (NCBI) | 4437 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P20585 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).
实验方案
Product Specific Protocols | |
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WB protocol for MSH3 antibody 82845-3-RR | Download protocol |
IF protocol for MSH3 antibody 82845-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |