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MRRF Polyclonal antibody

MRRF Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12357-2-AP

Print datasheet

Synonyms

MRFF, MRRF, MTRRF, RRF

验证数据展示

  • WB analysis using 12357-2-AP

    WB analysis using 12357-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12357-2-AP (MRRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12357-2-AP

    IHC staining of human pancreas cancer using 12357-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12357-2-AP (MRRF antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of MCF-7 using 12357-2-AP

    IF Staining of MCF-7 using 12357-2-AP

    Immunofluorescent analysis of MCF-7 cells, using MRRF antibody 12357-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12357-2-AP targets MRRF in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse, rat
Immunogen MRRF fusion protein Ag3021 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosome recycling factor
Synonyms MRFF, MRRF, MTRRF, RRF
Calculated Molecular Weight 262 aa, 29 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC013049
Gene Symbol MRRF
Gene ID (NCBI) 92399
RRIDAB_2144259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96E11
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRRF antibody 12357-2-APDownload protocol
IHC protocol for MRRF antibody 12357-2-APDownload protocol
IF protocol for MRRF antibody 12357-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biochem Biophys Res Commun

Muscle denervation reduces mitochondrial biogenesis and mitochondrial translation factor expression in mice.

Authors - Takumi Yokokawa
View Article
mouseWB

Physiol Rep

Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle.

Authors - Takumi Yokokawa
View Article

EMBO J

Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism

Authors - Ying Shu
View Article
ratWB

Sci Total Environ

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

Authors - Dongjie Li
View Article