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MRPS28 Polyclonal antibody

MRPS28 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16378-1-AP

Print datasheet

Synonyms

HSPC007, MRP S28, MRP S35, MRPS28, MRPS35, S28mt, S35mt

验证数据展示

  • WB analysis using 16378-1-AP

    WB analysis using 16378-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16378-1-AP (MRPS28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16378-1-AP

    IF Staining of HepG2 using 16378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16378-1-AP (MRPS28 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 16378-1-AP

    IHC staining of human liver using 16378-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16378-1-AP (MRPS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MRPS28 antibody included

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MRPS28 Ready-To-Use IHC Kit. MRPS28 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16378-1-AP targets MRPS28 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPS28 fusion protein Ag9354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S28
Synonyms HSPC007, MRP S28, MRP S35, MRPS28, MRPS35, S28mt, S35mt
Calculated Molecular Weight 187 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC010150
Gene Symbol MRPS28
Gene ID (NCBI) 28957
RRIDAB_2180748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Q9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS28 antibody 16378-1-APDownload protocol
IHC protocol for MRPS28 antibody 16378-1-APDownload protocol
IF protocol for MRPS28 antibody 16378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Authors - Juliette Pulman
View Article