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验证数据展示

  • WB analysis of mouse brain using 15907-1-AP

    WB analysis of mouse brain using 15907-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15907-1-AP (MRPS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 15907-1-AP

    IHC staining of human stomach using 15907-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 15907-1-AP (MRPS2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human stomach using 15907-1-AP

    IHC staining of human stomach using 15907-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 15907-1-AP (MRPS2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 15907-1-AP

    IF Staining of HepG2 using 15907-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPS2 antibody (15907-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MRPS2 Polyclonal antibody

MRPS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15907-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CGI 91, CGI91, CGI-91, COXPD36, MRP S2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15907-1-AP targets MRPS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8513

Product name: Recombinant human MRPS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-296 aa of BC008017

Sequence: MATSSAALPRILGAGARAPSRWLGFLGKATPRPARPSRRTLGSATALMIRESEDSTDFNDKILNEPLKHSDFFNVKELFSVRSLFDARVHLGHKAGCRHRFMEPYIFGSRLDHDIIDLEQTATHLQLALNFTAHMAYRKGIILFISRNRQFSYLIENMARDCGEYAHTRYFRGGMLTNARLLFGPTVRLPDLIIFLHTLNNIFEPHVAVRDAAKMNIPTVGIVDTNCNPCLITYPVPGNDDSPLAVHLYCRLFQTAITRAKEKRQQVEALYRLQGQKEPGDQGPAHPPGADMSHSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S2
别名 CGI 91, CGI91, CGI-91, COXPD36, MRP S2
计算分子量 296 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC008017
基因名称 MRPS2
Gene ID (NCBI) 51116
RRIDAB_10642944
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y399
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS2 antibody 15907-1-APDownload protocol
IHC protocol for MRPS2 antibody 15907-1-APDownload protocol
IF protocol for MRPS2 antibody 15907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols