MRPS15 Polyclonal antibody
MRPS15 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, MCF-7 cells, Raji cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 12 publications below |
产品信息
17006-1-AP targets MRPS15 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MRPS15 fusion protein Ag10702 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitochondrial ribosomal protein S15 |
| Synonyms | DC37, MPR S15, MRP S15, MRPS15, RPMS15, S15mt |
| Calculated Molecular Weight | 257 aa, 30 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC031336 |
| Gene Symbol | MRPS15 |
| Gene ID (NCBI) | 64960 |
| RRID | AB_2301068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P82914 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRPS15, also named as RPMS15 or DC37, is a 257 amino acid protein, which belongs to the ribosomal protein S15P family. MRPS15 localizes in the Mitochondrion and is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. MRPS15 is involved into the mitochondrial proteins biosynthesis and ribosomal biogenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPS15 antibody 17006-1-AP | Download protocol |
| IHC protocol for MRPS15 antibody 17006-1-AP | Download protocol |
| IF protocol for MRPS15 antibody 17006-1-AP | Download protocol |
| IP protocol for MRPS15 antibody 17006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Cell Metab Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. | ||
Hum Mol Genet Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement. |
