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验证数据展示

  • WB analysis using 28165-1-AP

    WB analysis using 28165-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28165-1-AP (MRPL39 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 28165-1-AP

    IHC staining of human liver using 28165-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28165-1-AP (MRPL39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 28165-1-AP

    IF Staining of HepG2 using 28165-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPL39 antibody (28165-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

MRPL39 Polyclonal antibody

MRPL39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28165-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MRP L39, Large ribosomal subunit protein mL39, L5mt, L39mt, COXPD59

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse heart tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

28165-1-AP targets MRPL39 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MRPL39 fusion protein Ag27154 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L39
别名 MRP L39, Large ribosomal subunit protein mL39, L5mt, L39mt, COXPD59
计算分子量 338 aa, 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC107719
基因名称 MRPL39
Gene ID (NCBI) 54148
RRIDAB_2881080
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYK5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL39 antibody 28165-1-APDownload protocol
IHC protocol for MRPL39 antibody 28165-1-APDownload protocol
IF protocol for MRPL39 antibody 28165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Genet

Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency

Authors - Shabnam Bakhshalizadeh
View Article
humanWB

Signal Transduct Target Ther

The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.

Authors - Xujun Liu
View Article
humanWB

Hum Mol Genet

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease

Authors - Sumudu S C Amarasekera
View Article