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验证数据展示

  • IHC staining of human kidney using 23870-1-AP

    IHC staining of human kidney using 23870-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23870-1-AP (MRPL33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MRPL33 antibody included
  • IF Staining of HepG2 using 23870-1-AP

    IF Staining of HepG2 using 23870-1-AP

    Immunofluorescent analysis of HepG2 cells using 23870-1-AP (MRPL33 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MRPL33 Polyclonal antibody

MRPL33 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 23870-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

C2orf1, L33mt, MRP L33, MRPL33, RPL33L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MRPL33 Ready-To-Use IHC Kit. MRPL33 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23870-1-AP targets MRPL33 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MRPL33 fusion protein Ag20921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L33
别名 C2orf1, L33mt, MRP L33, MRPL33, RPL33L
计算分子量 65 aa, 8 kDa
GenBank蛋白编号BC009475
基因名称 MRPL33
Gene ID (NCBI) 9553
RRIDAB_2879345
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO75394
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPL33 is a mitochondrial ribosomal protein. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion.

实验方案

Product Specific Protocols
IHC protocol for MRPL33 antibody 23870-1-APDownload protocol
IF protocol for MRPL33 antibody 23870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols