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MRPL33 Polyclonal antibody

MRPL33 Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 23870-1-AP

Print datasheet

Synonyms

C2orf1, L33mt, MRP L33, MRPL33, RPL33L

验证数据展示

  • IHC staining of human kidney using 23870-1-AP

    IHC staining of human kidney using 23870-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23870-1-AP (MRPL33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MRPL33 antibody included
  • IF Staining of HepG2 using 23870-1-AP

    IF Staining of HepG2 using 23870-1-AP

    Immunofluorescent analysis of HepG2 cells using 23870-1-AP (MRPL33 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MRPL33 Ready-To-Use IHC Kit. MRPL33 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23870-1-AP is MRPL33 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human
Immunogen MRPL33 fusion protein Ag20921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L33
Synonyms C2orf1, L33mt, MRP L33, MRPL33, RPL33L
Calculated Molecular Weight 65 aa, 8 kDa
GenBank Accession NumberBC009475
Gene Symbol MRPL33
Gene ID (NCBI) 9553
RRIDAB_2879345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO75394
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPL33 is a mitochondrial ribosomal protein. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion.

实验方案

Product Specific Protocols
IHC protocol for MRPL33 antibody 23870-1-APDownload protocol
IF protocol for MRPL33 antibody 23870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols