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验证数据展示

  • WB analysis using 21604-1-AP

    WB analysis using 21604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21604-1-AP (MRPL28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 21604-1-AP

    IHC staining of human kidney using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MRPL28 antibody included
  • IHC staining of human kidney using 21604-1-AP

    IHC staining of human kidney using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MRPL28 antibody included
  • IF Staining of HeLa using 21604-1-AP

    IF Staining of HeLa using 21604-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL28 antibody (21604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse brain using 21604-1-AP

    IP experiment of mouse brain using 21604-1-AP

    IP result of anti-MRPL28 (IP:21604-1-AP, 4ug; Detection:21604-1-AP 1:500) with mouse brain tissue lysate 4000ug.

MRPL28 Polyclonal antibody

MRPL28 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21604-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

L28mt, Large ribosomal subunit protein bL28m, MAAT1, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, JAR cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® MRPL28 Ready-To-Use IHC Kit. MRPL28 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21604-1-AP targets MRPL28 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag16224

Product name: Recombinant human MRPL28 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-256 aa of BC000990

Sequence: MPLHKYPVWLWKRLQLREGICSRLPGYYLRSLEEERTPTPVHYRPHGAKFKINPKNGQRERVEDVPIPIYFPPESQRGLWGGEGWILGQIYANNDKLSKRLKKVWKPQLFEREFYSEILDKKFTVTVTMRTLDLIDEAYGLDFYILKTPKEDLCSKFGMDLKRGMLLRLARQDPQLHPEDPERRAAIYDKYKEFAIPEEEAEWVGLTLEEAIEKQRLLEEKDPVPLFKIYVAELIQQLQQQALSEPAVVQKRASGQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L28
别名 L28mt, Large ribosomal subunit protein bL28m, MAAT1, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC000990
基因名称 MRPL28
Gene ID (NCBI) 10573
RRIDAB_10859785
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13084
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for MRPL28 antibody 21604-1-APDownload protocol
IHC protocol for MRPL28 antibody 21604-1-APDownload protocol
IP protocol for MRPL28 antibody 21604-1-APDownload protocol
WB protocol for MRPL28 antibody 21604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease

Authors - Sumudu S C Amarasekera
View Article
humanWB

J Ethnopharmacol

Elucidating the pharmacological effects of Compound Kushen injection on MYC-P15-CCND1 signaling pathway in nasopharyngeal carcinoma - An in vitro study

Authors - Zhishan Wu
View Article
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