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验证数据展示

  • WB analysis using 21604-1-AP

    WB analysis using 21604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21604-1-AP (MRPL28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 21604-1-AP

    IHC staining of human kidney using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 21604-1-AP

    IHC staining of human kidney using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 21604-1-AP

    IF Staining of HeLa using 21604-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL28 antibody (21604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse brain using 21604-1-AP

    IP experiment of mouse brain using 21604-1-AP

    IP result of anti-MRPL28 (IP:21604-1-AP, 4ug; Detection:21604-1-AP 1:500) with mouse brain tissue lysate 4000ug.

MRPL28 Polyclonal antibody

MRPL28 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21604-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

L28mt, MAAT1, Melanoma antigen p15, MRP L28, MRPL28, p15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, JAR cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21604-1-AP targets MRPL28 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag16224

Product name: Recombinant human MRPL28 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-256 aa of BC000990

Sequence: MPLHKYPVWLWKRLQLREGICSRLPGYYLRSLEEERTPTPVHYRPHGAKFKINPKNGQRERVEDVPIPIYFPPESQRGLWGGEGWILGQIYANNDKLSKRLKKVWKPQLFEREFYSEILDKKFTVTVTMRTLDLIDEAYGLDFYILKTPKEDLCSKFGMDLKRGMLLRLARQDPQLHPEDPERRAAIYDKYKEFAIPEEEAEWVGLTLEEAIEKQRLLEEKDPVPLFKIYVAELIQQLQQQALSEPAVVQKRASGQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L28
别名 L28mt, MAAT1, Melanoma antigen p15, MRP L28, MRPL28, p15
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC000990
基因名称 MRPL28
Gene ID (NCBI) 10573
RRIDAB_10859785
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13084
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL28 antibody 21604-1-APDownload protocol
IHC protocol for MRPL28 antibody 21604-1-APDownload protocol
IF protocol for MRPL28 antibody 21604-1-APDownload protocol
IP protocol for MRPL28 antibody 21604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease

Authors - Sumudu S C Amarasekera
View Article
humanWB

J Ethnopharmacol

Elucidating the pharmacological effects of Compound Kushen injection on MYC-P15-CCND1 signaling pathway in nasopharyngeal carcinoma - An in vitro study

Authors - Zhishan Wu
View Article