验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
16224-1-AP targets MRPL24 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9277 Product name: Recombinant human MRPL24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC016700 Sequence: MRLSALLALASKVTLPPHYRYGMSPPGSVADKRKNPPWIRRRPVVVEPISDEDWYLFCGDTVEILEGKDAGKQGKVVQVIRQRNWVVVGGLNTHYRYIGKTMDYRGTMIPSEAPLLHRQVKLVDPMDRKPTEIEWRFTEAGERVRVSTRSGRIIPKPEFPRADGIVPETWIDGPKDTSVEDALERTYVPCLKTLQEEVMEAMGIKETRKYKKVYWY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial ribosomal protein L24 |
| 别名 | MRP-L24, MRP L24, MRP L18, Large ribosomal subunit protein uL24m, L24mt |
| 计算分子量 | 216 aa, 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC016700 |
| 基因名称 | MRPL24 |
| Gene ID (NCBI) | 79590 |
| RRID | AB_2297885 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96A35 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL24 antibody 16224-1-AP | Download protocol |
| IP protocol for MRPL24 antibody 16224-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Biol Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. | ||
Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation | ||
J Biol Chem The Pseudouridine Synthase RPUSD4 is an essential component of Mitochondrial RNA Granules. | ||
Proteomes Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins. | ||
3 Biotech MRPL24 drives breast cancer metastasis and stemness by targeting c-MYC, BRD4, and STAT3 | ||