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验证数据展示

  • WB analysis using 16978-1-AP

    WB analysis using 16978-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16978-1-AP (MRPL21 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16978-1-AP

    IHC staining of human liver using 16978-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16978-1-AP (MRPL21 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16978-1-AP

    IHC staining of human liver using 16978-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16978-1-AP (MRPL21 antibody) at dilution of 1:100 (under 40x lens).

MRPL21 Polyclonal antibody

MRPL21 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16978-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

L21mt, MRP L21, MRPL21

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, HCT 116 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16978-1-AP targets MRPL21 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRPL21 fusion protein Ag9807 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L21
别名 L21mt, MRP L21, MRPL21
计算分子量 209 aa, 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC055088
基因名称 MRPL21
Gene ID (NCBI) 219927
RRIDAB_2145748
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z2W9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL21 antibody 16978-1-APDownload protocol
IHC protocol for MRPL21 antibody 16978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols