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MRPL21 Polyclonal antibody

MRPL21 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16978-1-AP

Print datasheet

Synonyms

L21mt, MRP L21, MRPL21

验证数据展示

  • WB analysis using 16978-1-AP

    WB analysis using 16978-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16978-1-AP (MRPL21 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16978-1-AP

    IHC staining of human liver using 16978-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16978-1-AP (MRPL21 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16978-1-AP

    IHC staining of human liver using 16978-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16978-1-AP (MRPL21 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inCOLO 320 cells, HCT 116 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16978-1-AP targets MRPL21 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen MRPL21 fusion protein Ag9807 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L21
Synonyms L21mt, MRP L21, MRPL21
Calculated Molecular Weight 209 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC055088
Gene Symbol MRPL21
Gene ID (NCBI) 219927
RRIDAB_2145748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z2W9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL21 antibody 16978-1-APDownload protocol
IHC protocol for MRPL21 antibody 16978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols