• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 14762-1-AP

    WB analysis using 14762-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14762-1-AP (MRPL16 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14762-1-AP

    IP experiment of HeLa using 14762-1-AP

    IP result of anti-MRPL16 (IP:14762-1-AP, 4ug; Detection:14762-1-AP 1:500) with HeLa cells lysate 1520 ug.

  • IHC staining of human intrahepatic cholangiocarcinoma using 14762-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 14762-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14762-1-AP (MRPL16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL16 Polyclonal antibody

MRPL16 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14762-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

PNAS-111, PNAS 111, MRP-L16, MRP L16, Large ribosomal subunit protein uL16m

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14762-1-AP targets MRPL16 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6523

Product name: Recombinant human MRPL16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-251 aa of BC001040

Sequence: MWRLLARASAPLLRVPLSDSWALLPASAGVKTLLPVPSFEDVSIPEKPKLRFIERAPLVPKVRREPKNLSDIRGPSTEATEFTEGNFAILALGGGYLHWGHFEMMRLTINRSMDPKNMFAIWRVPAPFKPITRKSVGHRMGGGKGAIDHYVTPVKAGRLVVEMGGRCEFEEVQGFLDQVAHKLPFAAKAVSRGTLEKMRKDQEERERNNQNPWTFERIATANMLGIRKVLSPYDLTHKGKYWGKFYMPKRV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L16
别名 PNAS-111, PNAS 111, MRP-L16, MRP L16, Large ribosomal subunit protein uL16m
计算分子量 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC001040
基因名称 MRPL16
Gene ID (NCBI) 54948
RRIDAB_3085442
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NX20
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPL16 (Mitochondrial Ribosomal Protein L16) is a protein coding gene. It encodes a 39S subunit protein. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. The molecular mass of MRPL16 is 28 kDa.

实验方案

Product Specific Protocols
IHC protocol for MRPL16 antibody 14762-1-APDownload protocol
IP protocol for MRPL16 antibody 14762-1-APDownload protocol
WB protocol for MRPL16 antibody 14762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...