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验证数据展示

  • IF Staining of HepG2 using 85109-4-RR

    IF Staining of HepG2 using 85109-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 85109-4-RR

    IF Staining of HeLa using 85109-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL11 antibody (85109-4-RR, Clone: 242768G1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 85109-4-RR

    FC experiment of HepG2 using 85109-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MRPL11 Recombinant antibody (85109-4-RR, Clone:242768G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 85109-4-RR

    Affinity and Kinetic Characterization of 85109-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85109-4-RR against Human MRPL11 were performed. The affinity constant is 14.5 nM.

MRPL11 Recombinant monoclonal antibody

MRPL11 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85109-4-RR

产品说明书

CloneNo. 242768G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

242768G1, CGI 113, CGI-113, L11mt, Large ribosomal subunit protein uL11m

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85109-4-RR targets MRPL11 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7899

Product name: Recombinant human MRPL11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-192 aa of BC005002

Sequence: MSKLGRAARGLRKPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDIKEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEIARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVKDLSSEELAAFQKERAIFLAAQKEADLAAQEEAAKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitochondrial ribosomal protein L11
别名 242768G1, CGI 113, CGI-113, L11mt, Large ribosomal subunit protein uL11m
计算分子量 21 kDa
GenBank蛋白编号BC005002
基因名称 MRPL11
Gene ID (NCBI) 65003
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y3B7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for MRPL11 antibody 85109-4-RRDownload protocol
IF protocol for MRPL11 antibody 85109-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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