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验证数据展示

  • WB analysis using 16652-1-AP

    WB analysis using 16652-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16652-1-AP (MRPL10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 16652-1-AP

    IHC staining of human testis using 16652-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16652-1-AP (MRPL10 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 16652-1-AP

    IHC staining of human testis using 16652-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16652-1-AP (MRPL10 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of mouse liver using 16652-1-AP

    IP experiment of mouse liver using 16652-1-AP

    IP result of anti-MRPL10 (IP:16652-1-AP, 4ug; Detection:16652-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

  • WB analysis of HeLa using 16652-1-AP

    WB analysis of HeLa using 16652-1-AP

    WB result of MRPL10 antibody (16652-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL10 transfected HeLa cells.

  • IF Staining of HepG2 using 16652-1-AP

    IF Staining of HepG2 using 16652-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPL10 antibody (16652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

MRPL10 Polyclonal antibody

MRPL10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16652-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

MRP-L10, MRP L8, MRP L10, Large ribosomal subunit protein uL10m, L8mt

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HEK-293 mitochondrial
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16652-1-AP targets MRPL10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MRPL10 fusion protein Ag10087 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L10
别名 MRP-L10, MRP L8, MRP L10, Large ribosomal subunit protein uL10m, L8mt
计算分子量 261 aa, 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC052601
基因名称 MRPL10
Gene ID (NCBI) 124995
RRIDAB_2282109
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z7H8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL10 antibody 16652-1-APDownload protocol
IHC protocol for MRPL10 antibody 16652-1-APDownload protocol
IF protocol for MRPL10 antibody 16652-1-APDownload protocol
IP protocol for MRPL10 antibody 16652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
View Article
humanWB

EMBO Rep

Mitochondrial calcium uniporter complex controls T-cell-mediated immune responses

Authors - Magdalena Shumanska
View Article