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验证数据展示

  • WB analysis using 25358-1-AP (same clone as 25358-1-PBS)

    WB analysis using 25358-1-AP (same clone as 25358-1-PBS)

    37°C incubated HeLa cells were subjected to SDS PAGE followed by western blot with 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 25358-1-AP (same clone as 25358-1-PBS)

    IHC staining of human colon using 25358-1-AP (same clone as 25358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 25358-1-AP (same clone as 25358-1-PBS)

    IHC staining of human colon using 25358-1-AP (same clone as 25358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 25358-1-AP (same clone as 25358-1-PBS)

    IHC staining of human liver using 25358-1-AP (same clone as 25358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

MRP3/ABCC3 Polyclonal antibody, PBS Only

MRP3/ABCC3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25358-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

ABCC3, MRP3, ABC31, ATP-binding cassette sub-family C member 3, EC:7.6.2.-

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25358-1-PBS targets MRP3/ABCC3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19786

Product name: Recombinant human ABCC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 838-960 aa of BC137348

Sequence: LLQRNGSFANFLCNYAPDEDQGHLEDSWTALEGAEDKEALLIEDTLSNHTDLTDNDPVTYVVQKQFMRQLSALSSDGEGQGRPVPRRHLGPSEKVQVTEAKADGALTQEEKAAIGTVELSVFW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family C (CFTR/MRP), member 3
别名 ABCC3, MRP3, ABC31, ATP-binding cassette sub-family C member 3, EC:7.6.2.-
计算分子量 1527 aa, 169 kDa
观测分子量 170-180 kDa
GenBank蛋白编号BC137348
基因名称 MRP3
Gene ID (NCBI) 8714
RRIDAB_3085789
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15438
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MRP3 also known as ABCC3, belongs to ATP-binding cassette (ABC) transporter protein superfamily. MRP3 hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants, and endogenous compounds across cell membranes (PMID: 11581266, 15083066). In normal liver, MRP3 was found present mainly in the bile ducts. In livers of patients with various forms of cholestasis, MRP3 levels were frequently increased in the proliferative cholangiocytes, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts( PMID: 11850532, 11283840). MRP3 is expressed in the liver, colon, pancreas, and, at a lower level, in the kidney (PMID:10094960). For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

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