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MRP3/ABCC3 Polyclonal antibody

MRP3/ABCC3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25358-1-AP

Print datasheet

Synonyms

ABC31, ABCC 3, ABCC3, MLP2, MRP 3, MRP3

验证数据展示

  • WB analysis using 25358-1-AP

    WB analysis using 25358-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 25358-1-AP

    IHC staining of human colon using 25358-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 25358-1-AP

    IHC staining of human colon using 25358-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 25358-1-AP

    IHC staining of human liver using 25358-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected in37°C incubated HeLa cells
Positive IHC detected inhuman colon tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25358-1-AP targets MRP3/ABCC3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen MRP3/ABCC3 fusion protein Ag19786 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 3
Synonyms ABC31, ABCC 3, ABCC3, MLP2, MRP 3, MRP3
Calculated Molecular Weight 1527 aa, 169 kDa
Observed Molecular Weight 170-180 kDa
GenBank Accession NumberBC137348
Gene Symbol ABCC3
Gene ID (NCBI) 8714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15438
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRP3 also known as ABCC3, belongs to ATP-binding cassette (ABC) transporter protein superfamily. MRP3 hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants, and endogenous compounds across cell membranes (PMID: 11581266, 15083066). In normal liver, MRP3 was found present mainly in the bile ducts. In livers of patients with various forms of cholestasis, MRP3 levels were frequently increased in the proliferative cholangiocytes, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts( PMID: 11850532, 11283840). MRP3 is expressed in the liver, colon, pancreas, and, at a lower level, in the kidney (PMID:10094960). For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

实验方案

Product Specific Protocols
WB protocol for MRP3/ABCC3 antibody 25358-1-APDownload protocol
IHC protocol for MRP3/ABCC3 antibody 25358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols