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MPPED1 Polyclonal antibody

MPPED1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13677-1-AP

Print datasheet

Synonyms

239AB, Adult brain protein 239, C22orf1, FAM1A, MPPED1

验证数据展示

  • WB analysis of mouse brain using 13677-1-AP

    WB analysis of mouse brain using 13677-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13677-1-AP (MPPED1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 13677-1-AP

    IHC staining of human pancreas using 13677-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 13677-1-AP (MPPED1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 13677-1-AP

    IHC staining of human pancreas using 13677-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 13677-1-AP (MPPED1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 13677-1-AP

    IF Staining of HeLa using 13677-1-AP

    Immunofluorescent analysis of Hela cells, using MPPED1 antibody 13677-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas tissue, human brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13677-1-AP targets MPPED1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen MPPED1 fusion protein Ag4672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name metallophosphoesterase domain containing 1
Synonyms 239AB, Adult brain protein 239, C22orf1, FAM1A, MPPED1
Calculated Molecular Weight 326 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC028035
Gene Symbol MPPED1
Gene ID (NCBI) 758
RRIDAB_10644336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15442
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MPPED1 antibody 13677-1-APDownload protocol
IHC protocol for MPPED1 antibody 13677-1-APDownload protocol
IF protocol for MPPED1 antibody 13677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols