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验证数据展示

  • WB analysis using 83397-5-RR

    WB analysis using 83397-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83397-5-RR

    WB analysis using 83397-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83397-5-RR (MPHOSPH9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83397-5-RR

    FC experiment of A431 using 83397-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MPHOSPH9 Recombinant antibody (83397-5-RR, Clone:240182D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83397-5-RR

    Affinity and Kinetic Characterization of 83397-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83397-5-RR against Human MPHOSPH9 were performed. The affinity constant is below 1 pM.

MPHOSPH9 Recombinant antibody

MPHOSPH9 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83397-5-RR

产品说明书

CloneNo. 240182D6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

MPP9, MPP 9, M-phase phosphoprotein 9, 240182D6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, rat brain tissue, NIH/3T3 cells, PC-12 cells, SH-SY5Y cells, mouse brain tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83397-5-RR targets MPHOSPH9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MPHOSPH9 fusion protein Ag22228 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 M-phase phosphoprotein 9
别名 MPP9, MPP 9, M-phase phosphoprotein 9, 240182D6
计算分子量 1031 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC130440
基因名称 MPHOSPH9
Gene ID (NCBI) 10198
RRIDAB_3671049
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ99550
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MPHOSPH9, also known as MPP9, is a protein phosphorylated during mitosis. MPHOSPH9 negatively regulates cilia formation by recruiting the CP110-CEP97 complex at the distal end of the mother centriole in ciliary cells. MPHOSPH9 is a centrosome component that localizes to the distal and proximal ends of two centrioles(PMID: 30375385).

实验方案

Product Specific Protocols
WB protocol for MPHOSPH9 antibody 83397-5-RRDownload protocol
FC protocol for MPHOSPH9 antibody 83397-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols