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验证数据展示

  • WB analysis using 32275-1-AP (same clone as 32275-1-PBS)

    WB analysis using 32275-1-AP (same clone as 32275-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32275-1-AP (MPHOSPH10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32275-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 32275-1-AP (same clone as 32275-1-PBS)

    IF Staining of A431 using 32275-1-AP (same clone as 32275-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MPP10 antibody (32275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32275-1-PBS in a different storage buffer formulation.

MPP10 Polyclonal antibody, PBS Only

MPP10 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 32275-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

MPHOSPH10

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32275-1-PBS targets MPP10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37602

Product name: Recombinant human MPHOSPH10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 391-654 aa of BC126389

Sequence: TAQKRPENSLLEETLHFDHAVRMAPVITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIYEQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVSNLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATDKKRERRKKKYQKRMKIKEKEKRRKLLEKSSVDQAGKYSKTVASEKLKQLTKTGKASFIKDEGKDKALKSSQAFFSKLQDQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein)
别名 MPHOSPH10
计算分子量 681 aa, 79 kDa
观测分子量105 kDa
GenBank蛋白编号BC126389
基因名称 MPHOSPH10
Gene ID (NCBI) 10199
RRIDAB_3742563
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO00566
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MPP10, also known as M phase phosphoprotein 10, is a component of the U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. It plays a crucial role in the early cleavages during pre-18S ribosomal RNA processing.

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