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验证数据展示

  • WB analysis using 32275-1-AP

    WB analysis using 32275-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32275-1-AP (MPHOSPH10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 32275-1-AP

    IF Staining of A431 using 32275-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MPP10 antibody (32275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MPP10 Polyclonal antibody

MPP10 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32275-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

MPHOSPH10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32275-1-AP targets MPP10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37602

Product name: Recombinant human MPHOSPH10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 391-654 aa of BC126389

Sequence: TAQKRPENSLLEETLHFDHAVRMAPVITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIYEQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVSNLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATDKKRERRKKKYQKRMKIKEKEKRRKLLEKSSVDQAGKYSKTVASEKLKQLTKTGKASFIKDEGKDKALKSSQAFFSKLQDQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein)
别名 MPHOSPH10
计算分子量 681 aa, 79 kDa
观测分子量105 kDa
GenBank蛋白编号BC126389
基因名称 MPHOSPH10
Gene ID (NCBI) 10199
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO00566
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MPP10, also known as M phase phosphoprotein 10, is a component of the U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. It plays a crucial role in the early cleavages during pre-18S ribosomal RNA processing.

实验方案

Product Specific Protocols
IF protocol for MPP10 antibody 32275-1-APDownload protocol
WB protocol for MPP10 antibody 32275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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