验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32275-1-AP targets MPP10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37602 Product name: Recombinant human MPHOSPH10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 391-654 aa of BC126389 Sequence: TAQKRPENSLLEETLHFDHAVRMAPVITEETTLQLEDIIKQRIRDQAWDDVVRKEKPKEDAYEYKKRLTLDHEKSKLSLAEIYEQEYIKLNQQKTAEEENPEHVEIQKMMDSLFLKLDALSNFHFIPKPPVPEIKVVSNLPAITMEEVAPVSVSDAALLAPEEIKEKNKAGDIKTAAEKTATDKKRERRKKKYQKRMKIKEKEKRRKLLEKSSVDQAGKYSKTVASEKLKQLTKTGKASFIKDEGKDKALKSSQAFFSKLQDQV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) |
| 别名 | MPHOSPH10 |
| 计算分子量 | 681 aa, 79 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC126389 |
| 基因名称 | MPHOSPH10 |
| Gene ID (NCBI) | 10199 |
| RRID | AB_3742563 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O00566 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MPP10, also known as M phase phosphoprotein 10, is a component of the U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. It plays a crucial role in the early cleavages during pre-18S ribosomal RNA processing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MPP10 antibody 32275-1-AP | Download protocol |
| WB protocol for MPP10 antibody 32275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
