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验证数据展示

  • WB analysis using 32307-1-AP

    WB analysis using 32307-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32307-1-AP (MOSPD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 32307-1-AP

    IHC staining of mouse cerebellum using 32307-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32307-1-AP (MOSPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MOSPD1 Polyclonal antibody

MOSPD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32307-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Motile sperm domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, PC-3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32307-1-AP targets MOSPD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MOSPD1 fusion protein Ag37532 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 motile sperm domain containing 1
别名 Motile sperm domain-containing protein 1
计算分子量 24 kDa
观测分子量25 kDa
GenBank蛋白编号BC005700
基因名称 MOSPD1
Gene ID (NCBI) 56180
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UJG1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MOSPD1, or Motile Sperm Domain-Containing Protein 1, is a protein that has been implicated in various biological processes. It is part of a family of proteins characterized by the presence of the major sperm protein domain and two transmembrane domains (PMID: 21792907). The protein is primarily located in the endoplasmic reticulum and Golgi apparatus, and it can also be found in the nucleus under certain conditions. MOSPD1 plays a significant role in the differentiation and proliferation of mesenchymal stem cells (MSCs). Studies have shown that MOSPD1-null embryonic stem cells exhibit impaired differentiation into various cell lineages, including osteoblasts and adipocytes, indicating its importance in MSC proliferation and differentiation (PMID: 26175344). Additionally, MOSPD1 is involved in the switch between mesenchymal and epithelial cells, suggesting its role in developmental regulation.

实验方案

Product Specific Protocols
WB protocol for MOSPD1 antibody 32307-1-APDownload protocol
IHC protocol for MOSPD1 antibody 32307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols