- Featured Product
- KD/KO Validated
MMP9 (N-terminal) Polyclonal antibody
MMP9 (N-terminal) Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
FC, IF, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
980
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HEK-293 cells, LNCaP cells, Raji cells, HepG2 cells, MDA-MB-231 cells |
Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
产品信息
10375-2-AP targets MMP9 (N-terminal) in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | CoIP, ELISA, IF, IHC, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, fish, hamster, pig, bovine |
Immunogen | MMP9 (N-terminal) fusion protein Ag0552 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) |
Synonyms | 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9, MMP-9, MMP9 (N-terminal) |
Calculated molecular weight | 707 aa, 78 kDa |
Observed molecular weight | 67 kDa, 92 kDa |
GenBank accession number | BC006093 |
Gene symbol | MMP9 |
Uniprot | P14780 |
Gene ID (NCBI) | 4318 |
RRID | AB_10897178 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for MMP9 (N-terminal) antibody 10375-2-AP | Download protocol |
IHC protocol for MMP9 (N-terminal) antibody 10375-2-AP | Download protocol |
IF protocol for MMP9 (N-terminal) antibody 10375-2-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1. | ||
Cell Host Microbe Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation | ||
Mol Cancer CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA. | ||
J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis. | ||
Sci Adv Self-powered enzyme-linked microneedle patch for scar-prevention healing of diabetic wounds |