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验证数据展示

  • IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

    IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP9 antibody included
  • IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

    IHC staining of human cervical cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP9 antibody included
  • WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP-9 (N-terminal) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 10375-2-AP (same clone as 10375-2-PBS)

    WB analysis of U2OS using 10375-2-AP (same clone as 10375-2-PBS)

    WB result of MMP-9 (N-terminal) antibody (10375-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP-9 (N-terminal) transfected U2OS cells. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP9 antibody included
  • IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    IHC staining of human breast cancer using 10375-2-AP (same clone as 10375-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP9 antibody included
  • FC experiment of HeLa using 10375-2-AP (same clone as 10375-2-PBS)

    FC experiment of HeLa using 10375-2-AP (same clone as 10375-2-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MMP9 (N-terminal) (10375-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    WB analysis using 10375-2-AP (same clone as 10375-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 10375-2-AP (same clone as 10375-2-PBS)

    IF Staining of HepG2 using 10375-2-AP (same clone as 10375-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP9 (N-terminal) antibody (10375-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10375-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MMP-9 (N-terminal) Polyclonal antibody, PBS Only

MMP-9 (N-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 10375-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

MMP9 (N-terminal), MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MMP9 Ready-To-Use IHC Kit. MMP9 primary antibody included.

产品信息

10375-2-PBS targets MMP-9 (N-terminal) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0552

Product name: Recombinant human MMP9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC006093

Sequence: MSLWQPLVLVLLVLGCCFAAPRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
别名 MMP9 (N-terminal), MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B
计算分子量 707 aa, 78 kDa
观测分子量92 kDa, 67 kDa
GenBank蛋白编号BC006093
基因名称 MMP-9
Gene ID (NCBI) 4318
RRIDAB_10897178
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14780
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

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