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验证数据展示

  • FC experiment of Transfected HEK-293T using 98361-3-RR

    FC experiment of Transfected HEK-293T using 98361-3-RR

    1x10^6 HEK-293T cells (left) or MMP8 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat MMP8 Rabbit RecAb (98361-3-RR, Clone: 242473B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using 98361-3-RR

    FC experiment of Transfected HEK-293T using 98361-3-RR

    1x10^6 HEK-293T cells (left) or MMP8 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat MMP8 Rabbit RecAb (98361-3-RR, Clone:242473B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Anti-Rat MMP8 Rabbit Recombinant Antibody

MMP8 Recombinant Antibody for FC (Intra)
Cat No. 98361-3-RR

产品说明书

CloneNo. 242473B12

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

FC (Intra)

别名

EC:3.4.24.34, Matrix metalloproteinase-8, MMP-8, Neutrophil collagenase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98361-3-RR targets MMP8 in FC (Intra) applications and shows reactivity with rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 8
别名 EC:3.4.24.34, Matrix metalloproteinase-8, MMP-8, Neutrophil collagenase
计算分子量53 kDa
GenBank蛋白编号NM_022221.1
基因名称 Mmp8
Gene ID (NCBI) 63849
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO88766
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487).

实验方案

Product Specific Protocols
FC protocol for MMP8 antibody 98361-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols