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验证数据展示

  • IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP3 antibody included
  • IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP3 antibody included
  • WB analysis of HeLa using 66338-1-Ig (same clone as 66338-1-PBS)

    WB analysis of HeLa using 66338-1-Ig (same clone as 66338-1-PBS)

    WB result of MMP3 antibody (66338-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP3 transfected HeLa cells. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • WB analysis using 66338-1-Ig (same clone as 66338-1-PBS)

    WB analysis using 66338-1-Ig (same clone as 66338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 66338-1-Ig (same clone as 66338-1-PBS)

    IHC staining of rat colon using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MMP3 antibody included
  • IF Staining of A549 using 66338-1-Ig (same clone as 66338-1-PBS)

    IF Staining of A549 using 66338-1-Ig (same clone as 66338-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (66338-1-Ig, Clone: 1F5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MMP3 Monoclonal antibody, PBS Only

MMP3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66338-1-PBS

产品说明书

CloneNo. 1F5A9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1F5A9, EC:3.4.24.17, Matrix metalloproteinase-3, MMP 3, MMP-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MMP3 Ready-To-Use IHC Kit. MMP3 primary antibody included.

产品信息

66338-1-PBS targets MMP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag12359

Product name: Recombinant human MMP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-477 aa of BC074869

Sequence: GEDTSMNLVQKYLENYYDLEKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 matrix metallopeptidase 3 (stromelysin 1, progelatinase)
别名 1F5A9, EC:3.4.24.17, Matrix metalloproteinase-3, MMP 3, MMP-3
计算分子量 477 aa, 54 kDa
观测分子量 45-60 kDa
GenBank蛋白编号BC074869
基因名称 MMP3
Gene ID (NCBI) 4314
RRIDAB_2881718
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08254
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

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