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验证数据展示

  • WB analysis of A549 using 18237-1-AP (same clone as 18237-1-PBS)

    WB analysis of A549 using 18237-1-AP (same clone as 18237-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 18237-1-AP (same clone as 18237-1-PBS)

    IHC staining of human kidney using 18237-1-AP (same clone as 18237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 18237-1-AP (same clone as 18237-1-PBS)

    IHC staining of human kidney using 18237-1-AP (same clone as 18237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 18237-1-AP (same clone as 18237-1-PBS)

    IF Staining of A549 using 18237-1-AP (same clone as 18237-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP28 antibody (18237-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18237-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MMP28 Polyclonal antibody, PBS Only

MMP28 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18237-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MM28, Matrix metalloproteinase-28, Matrix metalloproteinase 28, matrix metallopeptidase 28, Epilysin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18237-1-PBS targets MMP28 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12980

Product name: Recombinant human MMP28 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-393 aa of BC002631

Sequence: LGNAFDGPGGALAHAFLPRRGEAHFDQDERWSLSRRRGRNLFVVLAHEIGHTLGLTHSPAPRALMAPYYKRLGRDALLSWDDVLAVQSLYGKPLGGSVAVQLPGKLFTDFETWDSYSPQGRRPETQGPKYCHSSFDAITVDRQQQLYIFKGSHFWEVAADGNVSEPRPLQERWVGLPPNIEAAAVSLNDGDFYFFKVQSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 28
别名 MM28, Matrix metalloproteinase-28, Matrix metalloproteinase 28, matrix metallopeptidase 28, Epilysin
计算分子量 59 kDa
观测分子量 48 kDa
GenBank蛋白编号BC002631
基因名称 MMP28
Gene ID (NCBI) 79148
RRIDAB_2146444
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H239
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.

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