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MMP28 Polyclonal antibody

MMP28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18237-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

MM28, Matrix metalloproteinase-28, Matrix metalloproteinase 28, matrix metallopeptidase 28, Epilysin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, human colon tissue, mouse heart tissue
Positive IHC detected inhuman kidney tissue, human ovary tissue, human skin cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18237-1-AP targets MMP28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 MMP28 fusion protein Ag12980 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 28
别名 MM28, Matrix metalloproteinase-28, Matrix metalloproteinase 28, matrix metallopeptidase 28, Epilysin
计算分子量 59 kDa
观测分子量 48 kDa
GenBank蛋白编号BC002631
基因名称 MMP28
Gene ID (NCBI) 79148
RRIDAB_2146444
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H239
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.

实验方案

Product Specific Protocols
WB protocol for MMP28 antibody 18237-1-APDownload protocol
IHC protocol for MMP28 antibody 18237-1-APDownload protocol
IF protocol for MMP28 antibody 18237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Phytomedicine

Targeting the NF-κB p65-MMP28 axis: Wogonoside as a novel therapeutic agent for attenuating podocyte injury in diabetic nephropathy

Authors - Xiandeng Li
mouseIHC,IF

Cell Mol Life Sci

Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons.

Authors - Kyoung-In Cho
humanWB

FASEB J

Drastic induction of MMP-7 by cortisol in the human amnion: implications for membrane rupture at parturition.

Authors - Lu-Yao Wang
humanWB

Am J Pathol

CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2.

Authors - Zhang Min M
mouseWB,IF

Dis Model Mech

Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.

Authors - Kyoung-In Cho
humanIHC

Am J Pathol

Matrix Metalloproteinase-28 Is a Key Contributor to Emphysema Pathogenesis.

Authors - Anne M Manicone
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