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MMP21 Polyclonal antibody

MMP21 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 55289-1-AP

Print datasheet

Synonyms

matrix metallopeptidase 21, Matrix metalloproteinase 21, MMP 21, MMP21

验证数据展示

  • WB analysis of mouse liver using 55289-1-AP

    WB analysis of mouse liver using 55289-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55289-1-AP (MMP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 55289-1-AP

    IF Staining of L02 using 55289-1-AP

    Immunofluorescent analysis of L02 cells using 55289-1-AP (MMP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue, L02 cells
Positive IF detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55289-1-AP targets MMP21 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 21
Synonyms matrix metallopeptidase 21, Matrix metalloproteinase 21, MMP 21, MMP21
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberNM_147191
Gene Symbol MMP21
Gene ID (NCBI) 118856
RRIDAB_2881299
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N119
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP21 belongs to the peptidase M10A family. It may have an important and specific function in tumor progression and embryogenesis. It cleaves alpha-1-antitrypsin. The proenzyme without the signal peptide has a calculated molecular mass of 62 kD; following activation by furin, the processed enzyme has a calculated molecular mass of 49 kD(PMID:12617721),and MMP21 protein was detected in fetal neuroectoderm, in placenta, and in leukocytes(OMIM).This antibody is specific to MMP21.

实验方案

Product Specific Protocols
WB protocol for MMP21 antibody 55289-1-APDownload protocol
IF protocol for MMP21 antibody 55289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

Wnt activation disturbs cell competition and causes diffuse invasion of transformed cells through NF-κB-MMP21 pathway

Authors - Kazuki Nakai
View Article
humanWB

Hum Genomics

De novo disruptive heterozygous MMP21 variants are potential predisposing genetic risk factors in Chinese Han heterotaxy children

Authors - Xi-Ji Qin
View Article
human,mouseWB,IHC

Cancer Sci

Matrix metalloproteinase-21 promotes metastasis via increasing the recruitment and M2 polarization of macrophages in HCC

Authors - Jiangfan Zhou
  • KD Validated
View Article