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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83188-4-RR (same clone as 83188-4-PBS)

    WB analysis using 83188-4-RR (same clone as 83188-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83188-4-RR (MMP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83188-4-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83188-4-RR (same clone as 83188-4-PBS)

    FC experiment of MCF-7 using 83188-4-RR (same clone as 83188-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MMP13 Recombinant antibody (83188-4-RR, Clone:240060A1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83188-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83188-4-RR

    Affinity and Kinetic Characterization of 83188-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83188-4-RR against Human MMP13 were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

MMP13 Recombinant monoclonal antibody, PBS Only

MMP13 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83188-4-PBS

产品说明书

CloneNo. 240060A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

240060A1, CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83188-4-PBS targets MMP13 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34892

Product name: Recombinant human MMP13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 100-471 aa of BC074808

Sequence: DVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 matrix metallopeptidase 13
别名 240060A1, CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13
计算分子量 471 aa, 54 kDa
观测分子量 70 kDa, 54 kDa
GenBank蛋白编号BC074808
基因名称 MMP13
Gene ID (NCBI) 4322
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP45452
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated form (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).