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验证数据展示

  • WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

    WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

  • WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

    WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)

    IP experiment of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)

    IP result of anti-MMP13 (IP:18165-1-AP, 4ug; Detection:18165-1-AP 1:300) with MCF-7 cells lysate 2480 ug. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)

    IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)

    IHC staining of human liver using 18165-1-AP (same clone as 18165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)

    IF Staining of MCF-7 using 18165-1-AP (same clone as 18165-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (18165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

  • WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

    WB analysis using 18165-1-AP (same clone as 18165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18165-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MMP13 Polyclonal antibody, PBS Only

MMP13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18165-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13, Matrix metalloproteinase-13

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18165-1-PBS targets MMP13 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12653

Product name: Recombinant human MMP13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-471 aa of BC074808

Sequence: DVGEYNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 13
别名 CLG3, Collagenase 3, EC:3.4.24.-, Matrix metalloproteinase 13, Matrix metalloproteinase-13
计算分子量 471 aa, 54 kDa
观测分子量 65-70 kDa, 50-55 kDa, 40 kDa
GenBank蛋白编号BC074808
基因名称 MMP13
Gene ID (NCBI) 4322
RRIDAB_2144858
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP45452
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot(PMID:18332263).

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