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验证数据展示

  • WB analysis using 30805-1-AP

    WB analysis using 30805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30805-1-AP (MMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 30805-1-AP

    IHC staining of human cervical cancer using 30805-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30805-1-AP (MMP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MMP1 antibody included
  • IF Staining of HepG2 using 30805-1-AP

    IF Staining of HepG2 using 30805-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MMP1 antibody (30805-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MMP1 Polyclonal antibody

MMP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30805-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

22 kDa interstitial collagenase, 27 kDa interstitial collagenase, CLG, CLGN, EC:3.4.24.7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HepG2 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MMP1 Ready-To-Use IHC Kit. MMP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30805-1-AP targets MMP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MMP1 fusion protein Eg0527 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 matrix metallopeptidase 1 (interstitial collagenase)
别名 22 kDa interstitial collagenase, 27 kDa interstitial collagenase, CLG, CLGN, EC:3.4.24.7
计算分子量 54 kDa
观测分子量22-27 kDa, 42-52 kDa
GenBank蛋白编号BC013875
基因名称 MMP1
Gene ID (NCBI) 4312
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP03956
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1, 42-kDa is the activated MMP1(PMID:9418730).

实验方案

Product Specific Protocols
WB protocol for MMP1 antibody 30805-1-APDownload protocol
IHC protocol for MMP1 antibody 30805-1-APDownload protocol
IF protocol for MMP1 antibody 30805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols