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验证数据展示

  • WB analysis using 60600-1-Ig (same clone as 60600-1-PBS)

    WB analysis using 60600-1-Ig (same clone as 60600-1-PBS)

    Supernatant from non-treated and PMA treated U2OS cells were subjected to SDS PAGE followed by western blot with 60600-1-Ig (MMP-9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60600-1-PBS in a different storage buffer formulation.

  • Sample test of MP51000-1

    Sample test of MP51000-1

    Cytometric bead array of A549 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. A549 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Sample test of MP51000-1

    Sample test of MP51000-1

    Cytometric bead array of MM231 cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. MM231 cells were cultured in serum-free media overnight followed by PMA stimulation (200 nM, 24 h). 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Sample test of MP51000-1

    Sample test of MP51000-1

    Cytometric bead array of U2OS cells supernatant using MP51000-1, MMP-9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60600-1-PBS. Detection antibody: 60600-2-PBS. U2OS cells were cultured for 2 days in normal culture media or PMA (200nM, 48h) containing media. 50 μL of the supernatant was analyzed using MP51000-1 (MMP-9 Monoclonal Matched Antibody Pair). Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

MMP-9 Monoclonal antibody, PBS Only (Capture)

MMP-9 Monoclonal Antibody for WB, Indirect ELISA, Sample test
Cat No. 60600-1-PBS

产品说明书

CloneNo. 3C3E2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, Indirect ELISA, Sample test

别名

MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MMP9 Ready-To-Use IHC Kit. MMP9 primary antibody included.

产品信息

60600-1-PBS targets MMP-9 as part of a matched antibody pair:

MP51000-1: 60600-1-PBS capture and 60600-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 MMP-9 fusion protein Eg0442 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
别名 MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35
计算分子量 707 aa, 78 kDa
观测分子量 92 kDa
GenBank蛋白编号BC006093
基因名称 MMP-9
Gene ID (NCBI) 4318
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP14780
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling (PMID:7492685).