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验证数据展示

  • WB analysis using 60600-1-Ig

    WB analysis using 60600-1-Ig

    Supernatant from non-treated and PMA treated U2OS cells were subjected to SDS PAGE followed by western blot with 60600-1-Ig (MMP-9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MMP-9 Monoclonal antibody

MMP-9 Monoclonal Antibody for WB, ELISA
Cat No. 60600-1-Ig

产品说明书

CloneNo. 3C3E2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy MMP9 Ready-To-Use IHC Kit. MMP9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60600-1-Ig targets MMP-9 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 MMP-9 fusion protein Eg0442 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
别名 MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35
计算分子量 707 aa, 78 kDa
观测分子量 92 kDa
GenBank蛋白编号BC006093
基因名称 MMP-9
Gene ID (NCBI) 4318
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP14780
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling (PMID:7492685).

实验方案

Product Specific Protocols
WB protocol for MMP-9 antibody 60600-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols