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  • Featured Product
  • KD/KO Validated

MLN64 Polyclonal antibody

MLN64 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20292-1-AP

Print datasheet

Synonyms

CAB1, MLN 64, MLN64, Protein CAB1, STARD3

验证数据展示

  • WB analysis of MCF-7 using 20292-1-AP

    WB analysis of MCF-7 using 20292-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 20292-1-AP

    WB analysis of Jurkat using 20292-1-AP

    WB result of MLN64 antibody (20292-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLN64 transfected Jurkat cells.

  • IHC staining of human ovary tumor using 20292-1-AP

    IHC staining of human ovary tumor using 20292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human ovary tumor using 20292-1-AP

    IHC staining of human ovary tumor using 20292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 20292-1-AP (MLN64 antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of MCF-7 using 20292-1-AP

    IP experiment of MCF-7 using 20292-1-AP

    IP result of anti-MLN64 (IP:20292-1-AP, 3ug; Detection:20292-1-AP 1:500) with MCF-7 cells lysate 1200ug.

  • IF Staining of MCF-7 using 20292-1-AP

    IF Staining of MCF-7 using 20292-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20292-1-AP (MLN64 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 20292-1-AP

    WB analysis using 20292-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20292-1-AP (MLN64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF7 cells, mouse placenta tissue, MCF-7 cells, A549 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 20292-1-AP is MLN64 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MLN64 fusion protein Ag14061 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name StAR-related lipid transfer (START) domain containing 3
Synonyms CAB1, MLN 64, MLN64, Protein CAB1, STARD3
Calculated Molecular Weight 445 aa, 51 kDa
Observed Molecular Weight 33-53 kDa
GenBank Accession NumberBC008747
Gene Symbol STARD3
Gene ID (NCBI) 10948
RRIDAB_10643978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14849
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MLN64 (also known as STARD3) is an integral membrane protein anchored in late endosomes. MLN64 has a StAR-related lipid-transfer (START) domain to bind cholesterol, mediating intracellular cholesterol transfer. MLN64 is widely expressed in multiple tissues including liver, spleen, heart, kidney, lung, and the brain. Higher expression of MLN64 has been found in several malignancies. MLN64 can be detected as 33 kDa truncated fragment (PMID:9237999, PMID:15718238).

实验方案

Product Specific Protocols
WB protocol for MLN64 antibody 20292-1-APDownload protocol
IHC protocol for MLN64 antibody 20292-1-APDownload protocol
IF protocol for MLN64 antibody 20292-1-APDownload protocol
IP protocol for MLN64 antibody 20292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

PMID: 24905460

Authors - Rui Liu
View Article