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验证数据展示

  • WB analysis using 12062-1-AP (same clone as 12062-1-PBS)

    WB analysis using 12062-1-AP (same clone as 12062-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12062-1-AP (MLLT11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12062-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12062-1-AP (same clone as 12062-1-PBS)

    IHC staining of mouse brain using 12062-1-AP (same clone as 12062-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12062-1-AP (MLLT11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12062-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12062-1-AP (same clone as 12062-1-PBS)

    IHC staining of mouse brain using 12062-1-AP (same clone as 12062-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12062-1-AP (MLLT11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12062-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 12062-1-AP (same clone as 12062-1-PBS)

    IF Staining of SH-SY5Y using 12062-1-AP (same clone as 12062-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MLLT11 antibody (12062-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12062-1-PBS in a different storage buffer formulation.

MLLT11 Polyclonal antibody, PBS Only

MLLT11 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12062-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AF1Q, Protein AF1q, RP11 316M1.10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12062-1-PBS targets MLLT11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2699

Product name: Recombinant human MLLT11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of BC009624

Sequence: MRDPVSSQYSSFLFWRMPIPELDLSELEGLGLSDTATYKVKDSSVGKMIGQATAADQEKNPEGDGLLEYSTFNFWRAPIASIHSFELDLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 11
别名 AF1Q, Protein AF1q, RP11 316M1.10
计算分子量 90 aa, 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC009624
基因名称 MLLT11
Gene ID (NCBI) 10962
RRIDAB_3741822
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13015
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mixed-lineage leukemia translocated to 11 (MLLT11), also named ALL1-fused gene from the chromosome 1q (AF1q), which is located in chromosome l band 1q21, has been found to be overexpressed in ovarian cancer and to be associated with a poor patient outcome (PMID: 28423573). It is reported that MLLT11 is a pan-neuronal marker, suggesting a role in neural differentiation in the central nervous system and neural crest-cell derived peripheral ganglia (PMID: 24839873).

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