验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29278-1-AP targets KMT2A in WB, IF, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | KMT2A fusion protein Ag30468 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) |
| 别名 | MLL, HTRX, HRX, FLJ11783, CXXC7 |
| 计算分子量 | 432 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | NM_001197104 |
| 基因名称 | MLL |
| Gene ID (NCBI) | 4297 |
| RRID | AB_2918266 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q03164 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KMT2A, also known as mixed-lineage leukemia (MLL), is a gene that encodes a lysine methyltransferase responsible for the transcriptional activation through lysine 4 of histone 3 (H3K4) methylation. KMT2A is expressed mainly in the nucleus and is ubiquitously present in various tissues, particularly in ovary, lymph node, endometrium, thyroid, and brain tissue. It plays a significant role in embryonic development, hematopoiesis, and neurodevelopment. Mutations in KMT2A have been associated with several pathological conditions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KMT2A antibody 29278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics | ||
J Inflamm (Lond) Histone H3 posttranslational modified enzymes defined neutrophil plasticity and their vulnerability to IL-10 in the course of the inflammation | ||
Cancer Lett PARP1 interacts with WDR5 to enhance target gene recognition and facilitate tumorigenesis |