- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated MLKL Monoclonal antibody
MLKL Monoclonal Antibody for IF/ICC,IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse and More (1)
Applications
IF/ICC,IF-P and More (1)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3D4C6
验证数据展示
经过测试的应用
Positive IF detected in | human liver cancer tissue |
For other applications, we recommend the unconjugated version of this antibody, 66675-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
FC | See 1 publications below |
产品信息
CL488-66675 targets MLKL in IF, FC applications and shows reactivity with Human, Mouse samples.
Tested Applications | IF/ICC,IF-P |
Cited Applications | IF, FC |
Tested Reactivity | Human, Mouse |
Cited Reactivity | mouse |
Immunogen | MLKL fusion protein Ag15188 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | mixed lineage kinase domain-like |
Synonyms | FLJ34389, MLKL |
Calculated Molecular Weight | 471 aa, 54 kDa |
Observed Molecular Weight | 54 kDa |
GenBank Accession Number | BC028141 |
Gene Symbol | MLKL |
Gene ID (NCBI) | 197259 |
RRID | AB_2883366 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q8NB16 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily, has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 MLKL antibody CL488-66675 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Transl Med TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury |