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验证数据展示

  • WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MLKL antibody included
  • IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IHC staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MLKL antibody included
  • IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    IF Staining of human liver cancer using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66675-1-Ig (MLKL antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 66675-1-Ig (same clone as 66675-1-PBS)

    IHC staining of mouse skin using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66675-1-Ig (MLKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MLKL antibody included
  • WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    WB analysis using 66675-1-Ig (same clone as 66675-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66675-1-Ig (MLKL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66675-1-Ig (same clone as 66675-1-PBS)

    IF Staining of HepG2 using 66675-1-Ig (same clone as 66675-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MLKL antibody (66675-1-Ig, Clone: 3D4C6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66675-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MLKL Monoclonal antibody, PBS Only

MLKL Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66675-1-PBS

产品说明书

CloneNo. 3D4C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

3D4C6, hMLKL, Mixed lineage kinase domain-like protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MLKL Ready-To-Use IHC Kit. MLKL primary antibody included.

产品信息

66675-1-PBS targets MLKL in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15188

Product name: Recombinant human MLKL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-263 aa of BC028141

Sequence: MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQSLESSSGKSPLEISRFKVKNVKTGSASGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mixed lineage kinase domain-like
别名 3D4C6, hMLKL, Mixed lineage kinase domain-like protein
计算分子量 471 aa, 54 kDa
观测分子量35-40 kDa, 50-55 kDa
GenBank蛋白编号BC028141
基因名称 MLKL
Gene ID (NCBI) 197259
RRIDAB_2882029
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8NB16
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mixed lineage kinase domain like pseudokinase (MLKL), belongs to the protein kinase superfamily and has two MW of 54 and 30 kDa. MLKL plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. The 66675-1-Ig antibody recognizes 54 kDa MLKL monomer, 216 kDa MLKL tetramer, and a band around 45-50 kDa which are similar to papers published. (PMID: 31848291). MLKL can be detected as a 35kDa truncated isoform (PMID: 35965541).

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