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MLH1 Monoclonal antibody, PBS Only

MLH1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67350-1-PBS

产品说明书

CloneNo. 2F12C4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, rat

应用

WB, Indirect ELISA

COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


产品信息

67350-1-PBS targets MLH1 in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, rat
免疫原 MLH1 fusion protein Ag27723 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
别名 COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1
计算分子量 756 aa, 85 kDa
观测分子量 85-100 kDa, 40-45 kDa
GenBank蛋白编号BC006850
基因名称 MLH1
Gene ID (NCBI) 4292
RRIDAB_2918485
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40692
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.

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