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验证数据展示

  • WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13186-1-AP (same clone as 13186-1-PBS)

    IF Staining of HepG2 using 13186-1-AP (same clone as 13186-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 13186-1-AP (MLF1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    WB analysis using 13186-1-AP (same clone as 13186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

    IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

    IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

MLF1IP Polyclonal antibody, PBS Only

MLF1IP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13186-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CENP 50, CENP U, CENP U(50), CENP50, CENP-50

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13186-1-PBS targets MLF1IP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3858

Product name: Recombinant human MLF1IP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 243-418 aa of BC031520

Sequence: MKTSDIKELNIVLPEFEKTHLEHQQRIESKVCKAAIATFYVNVKEQFIKMLKESQMLTNLKRKNAKMISDIEKKRQRMIEVQDELLRLEPQLKQLQTKYDELKERKSSLRNAAYFLSNLKQLYQDYSDVQAQEPNVKETYDSSSLPALLFKARTLLGAESHLRNINHQLEKLLDQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 MLF1 interacting protein
别名 CENP 50, CENP U, CENP U(50), CENP50, CENP-50
计算分子量 418 aa, 48 kDa
观测分子量 46-50 kDa, 60 kDa
GenBank蛋白编号BC031520
基因名称 MLF1IP
Gene ID (NCBI) 79682
RRIDAB_10642941
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ71F23
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MLF1IP, also named as CENPU, ICEN24, KLIP1 and PBIP1, belongs to the CENP-U/AME1 family. MLF1IP plays an important role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Moreover, MLF1IP has been shown to be involved in tumorigenesis in several types of cancer including glioblastoma, prostate cancer and luminal breast cancer (PMID: 27378428). MLF1IP has 3 isoforms with the molecular mass of 21, 38 and 47 kDa.

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